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Unit Overview

Description

This unit is taken over two semesters and parts 1 (SCIE3326) and 2 (SCIE3327) must be completed to fulfil the requirements of the unit. SCIE3326 and SCIE3327 are assessed as one unit. The unit provides an advanced course in molecular biology and is designed to continue the training of students in this discipline. It stresses the use of molecular approaches and how they are applied to solve and investigate biological problems. Students learn how molecular biology has been used to advance understanding in areas of current interest in biology. The subject areas covered include a selection from the following: molecular biology of cancer; gene therapy; plant molecular biology; multigene families, molecular biology of bacterial and viral pathogens; RNA export from the nucleus, protein quality control; cloning vectors; interactomics; mechanisms of recombination; signature-tagged mutagenesis; in vivo expression technology; regulation of gene expression in prokaryotes and eukaryotes; molecular biology of sugar sensing, and the organisation of genes in different genomes. The techniques covered range from DNA cloning and sequencing to message level analysis and PCR amplification. Students become familiar with the uses of molecular biology in the bacterial, plant and animal sciences. Practicals include set laboratories, plus an advanced practical/project with tutorials.

Credit
12 points
Outcomes

Students gain an understanding of both theoretical and practical molecular biology and acquire central laboratory skills.

Assessment

Indicative assessments in this unit are as follows: (1) laboratory tests, laboratory book and laboratory report assessments; (2) mid-semester test on lecture material; and (3) final examination. Further information is available in the unit outline.



Student may be offered supplementary assessment in this unit if they meet the eligibility criteria.

Unit Coordinator(s)
Dr Thomas Martin
Unit rules
Prerequisites
SCIE2225 Molecular Biology
or BIOC2203 Biochemistry and Molecular Biology of the Cell
Advisable prior study
(MICR2204 Introductory Microbiology
and MICR2205 General and Applied Microbiology)
or MICR2207 Agricultural Microbiology.
BIOC2201 Biochemistry of the Cell
and BIOC2202 Biochemical Regulation of Cell Function are recommended but not essential.
Students with insufficient background are required to do extra reading.
Contact hours
lectures: 37 hours
labs: 70 hours
tutorials: 6 hours
workshops: 3 hours. For details see handout in the laboratory manual and first lecture.
Recommended
reading

Weaver, R. F. Molecular Biology, 4th edn: McGraw Hill 2007

Lodish, H. et al. Molecular Cell Biology, 6th edn: Freeman & Co 2008 (7th edn can be used as well) 

Ausubel, F. M. et al. Short Protocols in Molecular Biology, 5th edn: Wiley 2002

Sambrook, J. et al. Molecular Cloning: a Laboratory Manual, 3rd edn: CSH 2001

Suggested
alternate texts

Watson, J. D. et al. Molecular Biology of the Gene, 6th edn: CSHL Press 2008

Lewin, B. Genes IX, 9th edn: Jones and Bartlett Publishers 2008

  • The availability of units in Semester 1, 2, etc. was correct at the time of publication but may be subject to change.
  • All students are responsible for identifying when they need assistance to improve their academic learning, research, English language and numeracy skills; seeking out the services and resources available to help them; and applying what they learn. Students are encouraged to register for free online support through GETSmart; to help themselves to the extensive range of resources on UWA's STUDYSmarter website; and to participate in WRITESmart and (ma+hs)Smart drop-ins and workshops.
  • Unit readings, including any essential textbooks, are listed in the unit outline for each unit, one week prior the commencement of study. The unit outline will be available via the LMS and the UWA Handbook one week prior the commencement of study. Reading lists and essential textbooks are subject to change each semester. Information on essential textbooks will also be made available on the Essential Textbooks. This website is updated regularly in the lead up to semester so content may change. It is recommended that students purchase essential textbooks for convenience due to the frequency with which they will be required during the unit. A limited number of textbooks will be made available from the Library in print and will also be made available online wherever possible. Essential textbooks can be purchased from the commercial vendors to secure the best deal. The Student Guild can provide assistance on where to purchase books if required. Books can be purchased second hand at the Guild Secondhand bookshop (second floor, Guild Village), which is located on campus.
  • Contact hours provide an indication of the type and extent of in-class activities this unit may contain. The total amount of student work (including contact hours, assessment time, and self-study) will approximate 150 hours per 6 credit points.